Journal: Biology

Article Title: Assessing Antimicrobial Efficacy on Plastics and Other Non-Porous Surfaces: A Closer Look at Studies Using the ISO 22196:2011 Standard

doi: 10.3390/biology13010059

Figure Lengend Snippet: Literature on the different reported results of studies using the ISO 22196 standard.

Article Snippet: Medical devices (Silicone and polyurethane surfaces) , S. aureus ATCC 6538 E. coli ATCC 8739 S. epidermidis ATCC 43862 Serratia marcescens ATCC 35984 K. pneumoniae ATCC 13883 E. faecalis ATCC 47077 , Additional incubation temperatures , Test specimens were incubated at 4 °C, 22 °C and 35 °C , Log reduction and Reduction (%) , [ ] .

Techniques: Modification, Bacteria, Incubation, Suspension, Sterility, Saline, Activity Assay, Polymer, Concentration Assay, Sonication, Control

Journal:

Article Title: Evaluation of Use of a New Chromogenic Agar in Detection of Urinary Tract Pathogens

doi:

Figure Lengend Snippet: Quality control testing of CHROMagar Orientation used for the evaluation

Article Snippet: A single species was isolated in 176 samples; two species were isolated in 12 samples, and three species were isolated in 2 samples. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Test microorganism Mean colony count (CFU) ± SD with: P a Reference medium b CHROMagar Orientation c E. coli ATCC 25922 174 ± 98 166 ± 47 0.511 K. pneumoniae ATCC 13883 210 ± 77 230 ± 99 0.747 P. aeruginosa ATCC 27853 138 ± 15 128 ± 11 0.084 P. mirabilis ATCC 4630 110 ± 34 128 ± 43 0.132 S. aureus ATCC 25923 129 ± 69 132 ± 68 0.219 E. faecalis ATCC 19433 168 ± 69 174 ± 75 0.468 Open in a separate window a No significant differences were found between CHROMagar Orientation and the reference medium ( P > 0.05 [paired t test]). b The reference medium was TSA plus 5% defibrinated sheep blood.

Techniques: Control

Journal:

Article Title: Evaluation of Use of a New Chromogenic Agar in Detection of Urinary Tract Pathogens

doi:

Figure Lengend Snippet: Urine isolates presumptively identified on CHROMagar Orientation according to pigment reaction

Article Snippet: A single species was isolated in 176 samples; two species were isolated in 12 samples, and three species were isolated in 2 samples. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Test microorganism Mean colony count (CFU) ± SD with: P a Reference medium b CHROMagar Orientation c E. coli ATCC 25922 174 ± 98 166 ± 47 0.511 K. pneumoniae ATCC 13883 210 ± 77 230 ± 99 0.747 P. aeruginosa ATCC 27853 138 ± 15 128 ± 11 0.084 P. mirabilis ATCC 4630 110 ± 34 128 ± 43 0.132 S. aureus ATCC 25923 129 ± 69 132 ± 68 0.219 E. faecalis ATCC 19433 168 ± 69 174 ± 75 0.468 Open in a separate window a No significant differences were found between CHROMagar Orientation and the reference medium ( P > 0.05 [paired t test]). b The reference medium was TSA plus 5% defibrinated sheep blood.

Techniques: Incubation

Journal:

Article Title: Evaluation of Use of a New Chromogenic Agar in Detection of Urinary Tract Pathogens

doi:

Figure Lengend Snippet: Specific color reactions of microorganisms on CHROMagar Orientation. 1, P. mirabilis; 2, E. faecalis; 3, K. pneumoniae; 4, P. aeruginosa; 5, E. coli; 6, S. aureus.

Article Snippet: A single species was isolated in 176 samples; two species were isolated in 12 samples, and three species were isolated in 2 samples. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Test microorganism Mean colony count (CFU) ± SD with: P a Reference medium b CHROMagar Orientation c E. coli ATCC 25922 174 ± 98 166 ± 47 0.511 K. pneumoniae ATCC 13883 210 ± 77 230 ± 99 0.747 P. aeruginosa ATCC 27853 138 ± 15 128 ± 11 0.084 P. mirabilis ATCC 4630 110 ± 34 128 ± 43 0.132 S. aureus ATCC 25923 129 ± 69 132 ± 68 0.219 E. faecalis ATCC 19433 168 ± 69 174 ± 75 0.468 Open in a separate window a No significant differences were found between CHROMagar Orientation and the reference medium ( P > 0.05 [paired t test]). b The reference medium was TSA plus 5% defibrinated sheep blood.

Techniques:

Journal:

Article Title: Evaluation of Use of a New Chromogenic Agar in Detection of Urinary Tract Pathogens

doi:

Figure Lengend Snippet: Distribution of isolates among positive urine specimens

Article Snippet: A single species was isolated in 176 samples; two species were isolated in 12 samples, and three species were isolated in 2 samples. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Test microorganism Mean colony count (CFU) ± SD with: P a Reference medium b CHROMagar Orientation c E. coli ATCC 25922 174 ± 98 166 ± 47 0.511 K. pneumoniae ATCC 13883 210 ± 77 230 ± 99 0.747 P. aeruginosa ATCC 27853 138 ± 15 128 ± 11 0.084 P. mirabilis ATCC 4630 110 ± 34 128 ± 43 0.132 S. aureus ATCC 25923 129 ± 69 132 ± 68 0.219 E. faecalis ATCC 19433 168 ± 69 174 ± 75 0.468 Open in a separate window a No significant differences were found between CHROMagar Orientation and the reference medium ( P > 0.05 [paired t test]). b The reference medium was TSA plus 5% defibrinated sheep blood.

Techniques:

Journal: Cancer Science

Article Title: Polarity Gene PARD6B Promotes Tumor Growth of Colorectal Cancer via Increasing MYC Expression

doi: 10.1111/cas.70119

Figure Lengend Snippet: PARD6B is specifically expressed in CRC cells and a potential therapeutic target. (A) t‐SNE of 12 annotated cell types. (B) PARD6B expression in t‐SNE plots. (C) PARD6B expression in normal epithelial vs. malignant CRC cells. *** p < 0.001. (D) Statistical analysis of 63 polarity‐associated genes in TCGA. X‐axis: Log2‐fold change (tumor vs. normal); Y‐axis: Pearson correlation (copy number vs. mRNA). Red dots: Genes linked to poor prognosis ( p < 0.05). (E) Analysis of 63 polarity genes; Green dots: Six Par family genes (PARD6B, PARD3, PRKCI, PRKCZ, RAC1, CDC42). (F) Kaplan–Meier overall survival curves by mRNA expression of five Par family genes above (excluding PARD6B) in TCGA CRC patients.

Article Snippet: Primary antibodies included PARD6B (sc‐166405, Santa Cruz), PKC iota (PRKCI) (13883‐1‐AP, Proteintech), β‐actin (sc‐47778, Santa Cruz), c‐MYC (ab32072, Abcam), E‐cadherin (#610181, BD Transduction Laboratories), and N‐cadherin (5D5, Abcam), all at 1:1000 dilution.

Techniques: Expressing

Journal: Cancer Science

Article Title: Polarity Gene PARD6B Promotes Tumor Growth of Colorectal Cancer via Increasing MYC Expression

doi: 10.1111/cas.70119

Figure Lengend Snippet: PARD6B promotes MYC expression via miR‐34c repression without altering EMT‐related gene expression. (A) Western blot of EMT‐related proteins in PARD6B knockout (KO) and overexpressing CRC cells. (B) GSEA of MYC‐associated gene signature by PARD6B expression in TCGA. (C) Analysis of 234 MYC‐associated genes in TCGA. X‐axis: Pearson correlation with PARD6B; Y‐axis: Log2 ( p ‐value). (D) Correlation between PARD6B and MYC mRNA expression in TCGA. (E) Correlations between PARD6B and miR‐34c, and between miR‐34c and MYC, using CCLE data (Pearson's R ). (F) Normalized miR‐34c relative expression in control vs. PARD6B KO or overexpression cells (mean ± SE). (G) Normalized MYC mRNA expression in PARD6B KO and overexpressing CRC cells. (H) Western blot of PARD6B, PRKCI, c‐MYC, and β‐action in NT vs. KO (HT29, CACO‐2) and control vs. PARD6B‐overexpressing (RKO, LS174T) cells. KO, knockout; NT, non‐target.

Article Snippet: Primary antibodies included PARD6B (sc‐166405, Santa Cruz), PKC iota (PRKCI) (13883‐1‐AP, Proteintech), β‐actin (sc‐47778, Santa Cruz), c‐MYC (ab32072, Abcam), E‐cadherin (#610181, BD Transduction Laboratories), and N‐cadherin (5D5, Abcam), all at 1:1000 dilution.

Techniques: Expressing, Gene Expression, Western Blot, Knock-Out, Control, Over Expression